Supplied in glycerol buffer

This product is for use when more severe EAE is desired. (More severe than the EAE obtained with our EAE induction kits.) For more severe EAE, increase the PTX amount by 20-50% above the dose in the recommended EAE induction protocol.

Additional PTX is not required for normal EAE induction; our EAE induction kits supply enough PTX for most labs. If you are having difficulty inducing EAE with Hooke Kits™, please contact us before ordering additional PTX. (Also see our recommended protocol for your kit for tips and troubleshooting.)

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Detailed contents

Pertussis toxin (PTX) from Bordetella pertussis, in glycerol buffer, 1 vial.

Each vial contains enough PTX to induce EAE in at least 10 mice.

Because PTX potency varies considerably by lot, each vial contains:

Pertussis toxin: 3 to 6 µg (varies by lot)
Concentration: 0.1 to 0.2 mg/mL (varies by lot)
Total volume: 25 to 30 µL (varies by lot)

Lot-specific details (PTX amount, concentration, volume) are supplied with the product.

Turbidty: Translucent
Form: Solution
Color: None, clear

Product selection

We recommend this product as an adjuvant for EAE induction in C57BL/6 mice. This is the same PTX supplied with our EAE induction kits for C57BL/6 mice.

References

Munoz JJ et al, Cell Imunol 83:92 (1984)
Janson L et al, Eur J Immunol 21:693 (1991)
Kamradt T, et al, J Immunol 147:3296 (1991)
Mendel et al, Eur J Immunol 25:1951 (1995)

Safety Data Sheet (SDS)

(PDF)

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