Supplied in pre-filled syringes, ready to use.
CIA is induced by chicken or bovine type II collagen in CFA, followed by a type II collagen booster 18 to 21 days later. The collagen booster is usually administered in IFA. Alternatively, the booster can be administered in CFA, resulting in faster CIA development and increased severity.
When used with the recommended protocol, 90 to 100% of mice develop arthritis within 35 days of immunization. Most mice reach disease severity of 10 to 14 (on the scale 0 to 16).
Each lot is tested and individually adjusted to ensure consistent disease induction.
Properly prepared emulsions are critical for reliable induction of CIA. Our emulsions are carefully made and pre-filled into syringes, ready to use, to reduce time needed to set up experiments.
Lot-to-lot reagent variations can cause dramatic changes in severity of induced autoimmune disease. The consistent potency of pre-characterized Hooke Kits™ eliminates time-consuming testing.
Pre-filled syringes are prepared under aseptic conditions and delivered in sterilized plastic bags for easy disinfection before introduction into your mouse facility.
Collagen-induced arthritis (CIA) is one of the most commonly used animal models of human rheumatoid arthritis (RA).
Joint inflammation, which develops in animals with CIA, strongly resembles inflammation observed in patients with RA. Blocking tumor necrosis factor (TNF) is efficacious in treatment of CIA, just as it is a highly efficacious therapy in treatment of RA patients.
CIA is mediated by both T cells and antibodies (B cells). Macrophages are believed to play an important role in mediating tissue damage during disease development.
CIA is induced by immunization with type II collagen/CFA emulsion, followed 18 to 21 days later by collagen/IFA or collagen/CFA emulsion. See recommended protocol.
While both bovine type II and chicken type II collagen are used for CIA induction, bovine collagen appears to be slightly better at inducing CIA in male DBA/1 mice, while chicken collagen works slightly better in females.
These kits can be customized for a small additional charge. Contact us at with your requirements.
Each kit provides sufficient emulsion for 20 mice.
Antigen is chicken type II collagen (EK-0210, EK-0211) or bovine type II collagen (EK-0220, EK-0221).
| Qty | Description |
|---|---|
| 2 |
Syringes pre-filled with 0.5 mL chicken type II collagen emulsion/CFA ~ 1 mg chicken type II collagen/mL emulsion ~ 1 mg killed mycobacterium tuberculosis H37Ra/mL emulsion |
| 1 | Data sheet: Recommended experimental protocol, typical results |
| Qty | Description |
|---|---|
| 2 |
Syringes pre-filled with 0.5 mL chicken type II collagen emulsion/IFA ~ 1 mg chicken type II collagen/mL emulsion |
| 1 | Data sheet: Recommended experimental protocol, typical results |
| Qty | Description |
|---|---|
| 2 |
Syringes pre-filled with 0.5 mL bovine type II collagen emulsion/CFA ~ 1 mg bovine type II collagen/mL emulsion ~ 1 mg killed mycobacterium tuberculosis H37Ra/mL emulsion |
| 1 | Data sheet: Recommended experimental protocol, typical results |
| Qty | Description |
|---|---|
| 2 |
Syringes pre-filled with 0.5 mL bovine type II collagen emulsion/IFA ~ 1 mg bovine type II collagen/mL emulsion |
| 1 | Data sheet: Recommended experimental protocol, typical results |
Stuart JM et al, JCI 69:673 (1982)
Holmdahl R et al, Clin Exp Immunol 62:639 (1985)
Chicken Collagen/CFA Emulsion (PDF)
Chicken Collagen/IFA Emulsion (PDF)
Bovine Collagen/CFA Emulsion (PDF)
Bovine Collagen/IFA Emulsion (PDF)
CK-0210 Hooke Control Kit™ for EK-0210
CK-0211 Hooke Control Kit™ for EK-0211
CK-0220 Hooke Control Kit™ for EK-0220
CK-0221 Hooke Control Kit™ for EK-0221
Tissue from mice with CIA
Serum from mice with CIA