Snap frozen tissue, fresh cells, and frozen serum from naïve mice (no immunization) and control mice (immunized with adjuvant only).
These products are for use as negative controls, for comparison to disease-induced mice. Tissue is time-matched to tissue from diseased mice.
Save time establishing & running disease models.
Contact for pricing and details.
Mice 8 to 14 weeks of age are acclimated to our facility for at least 7 days.
For products from immunized mice (does not apply to products from naïve mice), mice are then immunized with adjuvant(s) only as follows:
| Mouse strain | Hooke Control Kit(s)™ used for immunization | Using protocol |
|---|---|---|
| DBA/1 (CIA controls) | CK-0210 & CK-0211 (CFA & IFA emulsions) | CIA Induction in DBA/1 Mice |
| C57BL/6 (EAE controls) | CK-2110 (CFA emulsion) | EAE Induction by Active Immunization in C57BL/6 Mice |
| SJL (EAE controls) | CK-0120 (CFA emulsion) | EAE Induction by Active Immunization in SJL Mice |
Mice are euthanized at the timepoints given above.
If serum is included, blood is collected immediately after termination and serum is isolated.
All tissue is snap frozen in liquid nitrogen and stored at -80 °C until shipment.
Cell suspensions are prepared and red blood cells in spleen are lysed.
Cells are resuspended in complete tissue culture media and cell concentrations are determined and marked on tubes.
Cells are shipped the same day for overnight delivery.
At termination blood is immediately collected, and serum is isolated.
Serum from multiple mice is pooled and stored at -80 °C until shipment.
Each product unit contains tissue and/or serum from a single mouse.
Each organ is supplied in a separate 1.5 mL cryogenic tube. Tubes are marked with a mouse ID, paw ID (left or right hind paw; if applicable), and tissue weight.
Typical tissue quantity:
Each product unit contains the entire suspension of spleen and/or lymph node cells from one mouse.
Cells are supplied in 15 or 50 mL centrifuge tubes, in complete tissue culture media (90% RPMI 1640, 10% fetal calf serum, supplemented with 0.1 mM MEM non-essential amino acids, 1 mM MEM sodium pyruvate, 2 mM L-glutamine, 100 UI/mL penicillin, 100 µg/mL streptomycin, 5 x 10-5 M 2-mercaptoethanol).
Each cell type is supplied in a separate centrifuge tube, together with cell counts (cells/mL) for the cell type. If cells from multiple mice (of the same catalog number) are ordered, each type of cell will be pooled in one tube with one cell count.
(Please specify if you want separate tubes for each mouse instead of pooled cells; in that case the "first mouse" price applies.)
The typical number of cells expected for naïve and immunized mice is as follows:
| Cell type | Cells/mouse (immunized) |
Cells/mouse (naïve) |
|---|---|---|
| Splenocytes | 100 to 200 x 106 | 50 to 100 x 106 |
| Lymph node cells | 10 to 30 x 106 | 1 to 3 x 106 |
Frozen serum, pooled from multiple mice, supplied in a 1.5 mL cryogenic tube on dry ice.
Tissue from mice with CIA
Serum from mice with CIA
Tissue from mice with EAE
Control and naïve tissue, cells, and serum